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Wheat Germ Supplementation Alleviates Insulin Resistance and Cardiac Mitochondrial Dysfunction in an Animal Model of Diet-Induce

Wheat Germ Supplementation Alleviates Insulin Resistance and Cardiac Mitochondrial Dysfunction in an Animal Model of Diet-Induce

Name:
Babajide Ojo

Department:
Nutritional Sciences

Abstract:
Obesity is strongly associated with insulin resistance, along with mitochondrial dysfunction to metabolically active tissues and increased production of reactive oxygen species (ROS). Foods such as wheat germ (WG), which is rich in antioxidants, are thought to protect tissues from damage due to ROS and modulate some negative effects of obesity. This study examined the effects of WG supplementation on insulin resistance, mitochondrial substrate metabolism and innate antioxidant markers in the liver and heart of C57BL/6 mice fed a high fat (HF) diet. Six-week-old male C57BL/6 mice were randomized into four dietary treatment groups (n=12 mice/group): control (C, 10% fat kcal), control + 10% WG (C+WG), high fat (HF, 60% fat kcal), or HF+WG. After 90 days of treatment, HF+WG mice had significantly less visceral fat (-11%, P=0.0012) and were less hyperinsulinemic (P=0.0091) and less insulin resistant (-57%, P=0.011) compared to the HF group. Addition of WG to the control diet showed no considerable effect. HF feeding compared to control significantly elevated (45%, P=0.008) cardiac mitochondrial oxygen consumption in complex 2, suggesting increased metabolic stress, while WG supplementation in the HF diet stabilized this effect to the level of control. Consequently, Sod2 and Pgc1a genes which mediate antioxidant defense and mitochondrial biogenesis respectively, were significantly reduced (p