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Detection and Extraction of Shiga-toxigenic Escherichia coli specific Bacteriophage Depolymerase Enzyme

Detection and Extraction of Shiga-toxigenic Escherichia coli specific Bacteriophage Depolymerase Enzyme

Name:
Beata Mackenroth

Department:
Animal Science

Abstract:
Shiga-toxigenic Escherichia coli (STEC) are one of the major foodborne pathogens and have been associated with multiple foodborne outbreaks in the United States. These STEC can form biofilms on food and food processing equipment which are difficult for conventional sanitizers to remove. One potential means of degrading these biofilms is through the use of STEC specific bacteriophage depolymerase enzymes. In this study, depolymerase coding genes were detected via PCR, and crude enzyme extraction was performed. Crude enzyme extract was tested for inhibition of STEC biofilms in-vitro. Our results showed that depolymerase coding gene was present in the tested phage. The crude enzyme extract showed inhibition against STEC biofilms. Bacteriophage produced depolymerase enzymes could be utilized to reduce STEC biofilms in food industry.