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Chlamydia trachomatis manipulation of Protein Kinase C

Chlamydia trachomatis manipulation of Protein Kinase C

Name:
Prakash Sah

Department:
Microbiology and Molecular Genetics

Abstract:
Chlamydia trachomatis is responsible for causing a range of diseases such as blinding trachoma and urogenital infections leading to serious complications. Inside a host cell, C. trachomatis lives in a parasitophorous vacuole called an inclusion from where it is able to secrete various effectors to manipulate host-cellular functions to its benefit. Currently, not much is known about Chlamydial manipulation of host kinases such as Protein Kinase C (PKC). PKCs are members of AGC family of kinases and involved in regulating various cellular functions such as, growth and proliferation, migration, survival and apoptosis. We hypothesize that C. trachomatis manipulates PKC pathways to regulate intracellular development inside the host, as PKCs are important in regulating various cellular functions. Indirect immunofluorescence of infected cells verified recruitment of multiple PKC isoenzymes to microdomains (Src-family kinases rich regions) on the inclusion. Recruitment of PKC substrates, including Marcks, was also confirmed. Inhibition of PKC activity with Staurosporine at various time points resulted in decreased recoverable infectious progeny. These results confirm PKCs are important for intracellular growth and development of C. trachomatis.