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Screening sorghum lines for resistance against sugarcane aphid, Melanaphis sacchari (Zehnter)

Screening sorghum lines for resistance against sugarcane aphid, Melanaphis sacchari (Zehnter)

Name:
Sulochana Paudyal

Department:
Entomology & Plant Pathology

Abstract:
The sugarcane aphid Melanaphis sacchari (Zehnter) has emerged as the most significant threat to sorghum (Sorghum bicolor (L.) Moench) production in the United States. Since 2013, discovery of aphid resistant germplasm has been a priority all stakeholders involved. We screened twenty three different sorghum lines for resistance in a free choice assay by infesting entries with sugarcane aphids at the 3-4 leaf. Damage ratings were initiated when the susceptible control (585) was estimated to be 85% dead. All plants were evaluated using a damage rating of 1–9 (little to high damage). Chlorophyll loss was also quantified in a no-choice test on infested and uninfested leaves of each of the lines using SPAD-502 chlorophyll meter. Based on plant height, number of leaves, and chlorophyll values from the free-choice study, sorghum lines 0L0030, 95207, G1213, GW1489, 97157, TX 2783, W 7431, W844-E, and DKS-37-07 were categorized as highly resistant with rating ≈3, indicating that good sources of sugarcane aphid resistance are available. In particular, Chlorophyll loss in the susceptible check (585) was 80%, however, in the above resistant lines the loss figures were