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Antibiotic Resistance of Pseudomonas aeruginosa Recovered From Cystic Fibrosis Patients

Antibiotic Resistance of Pseudomonas aeruginosa Recovered From Cystic Fibrosis Patients

Name:
William Starr

Department:
Microbiology and Molecular Genetics

Abstract:
Cystic Fibrous (CF) patients produce dehydrated thick mucus in their lungs and lack the ability to clear this mucus due to mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR gene). The mucus provides an optimal environment for bacterial infections. It is reported that P. aeruginosa infects up to 50% of children and 80% of adults with CF. Once the infection has been acquired, eradication of P. aeruginosa from the CF lung is rare. P. aeruginosa is naturally resistant to many antibiotics and acquires antibiotic resistance during the infection process. This study aims to determine resistance profiles of P. aeruginosa clinical isolates from patients of various ages. Kirby-Bauer tests were performed on 52 isolates using nine different antibiotics which represent multiple antibiotic classes. To determine if resistance was due to genetic factors genomic DNA was extracted from the CF isolates and PCR was performed to verify the presence of eight antibiotic resistance genes. The results showed that all of the isolates had resistance to at least one of the nine antibiotics; however, not all of isolates showed the presence antibiotic resistance genes by PCR. The results also showed at higher dosing of antibiotics is needed for CF patients due to infections being able to survive the immune system, smaller antibiotic treatments, and swapping of genetic material between bacterial species. By understanding antibiotic resistance of P. aeruginosa from CF patients regards to the mechanisms in which this resistance is acquired , treatment options for CF patients can be more specialized and targeted based on age, infection type, and susceptibility or resistance to certain antibiotics.